Product Description
Introduction
The yield of plasmid py number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 3 to 5 times. Please contact our customer service for further information and reference the table below for the commonly used plasmids.
|
Plasmid |
Origin |
High copy |
Low copy |
|
pACYC |
P15A |
|
10-12 |
|
pSC101 |
pSC101 |
|
5 |
|
pSuperCos |
pMB1 |
DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high co |
10-20 |
|
pBR322 |
pMB1 |
|
15-20 |
|
pUC |
Muted pMB1 |
500-700 |
|
|
pGEMR |
Muted pMB1 |
300-400 |
|
|
pBluescriptR |
ColE1 |
300-500 |
|
Materials supplied by user
- Centrifuge with swing-bucket rotor (4,000 x g).
- Please disinfect 96-well 2.2 mL plates before use.
- Vacuum pump capable of achieving 300-400 mbar.
- Standard vacuum manifold.
- Oven or incubator preset to 70 oC.
Storage and Stability
Semua komponen dijamin selama 24 bulan sejak tanggal pembelian. Buffer A1 / RNase A harus disimpan pada 4oC.
Kit Contents
| Catalog# |
PD1812-S |
PD1812-01 |
PD1812-02 |
| Preps |
1 |
4 |
20 |
| 96- Well 2.2 mLPlate |
1 |
4 |
20 |
| 96- Well 1.6 mLPlate（Can be reused） |
1 |
4 |
20 |
| 96-Well DNA Plate |
1 |
4 |
20 |
| 96-Well Lysate Clearance Plate |
1 |
4 |
20 |
| 96-Well Collection Plate |
2 |
5 |
24 |
| Ventilating Film |
1 |
4 |
20 |
| Sealing Film |
4 |
16 |
80 |
| Buffer A1 |
30 mL(Add RNase A before use) |
110 mL(Add RNase A before use) |
2 x 300 mL |
| Buffer B1 |
30 mL(Keep tightly capped after use) |
110 mL(Keep tightly capped after use) |
2 x 300 mL |
| Buffer N1 |
40 mL(Contain chaotropic salts) |
160 mL(Contain chaotropic salts) |
2 x 400 mL |
| DNA Wash Buffer |
50 mL(Add 200 mL ethanol before use) |
2×100 mL(Add 400 mL ethanol before use) |
7 x125 mL(Add 500 mL ethanol before use) |
| Buffer KB |
55 mL |
240 mL |
3 x 400 mL |
| Elution Buffer |
25 mL |
100 mL |
450 mL |
| RNase A(20mg/mL) |
160 μL |
600 μL |
2 x 1.5 mL |
| Use Manual |
1 |
1 |
1 |
Before Starting
Ujilah buku pegangan ini dan kenali setiap langkahnya. Siapkan semua komponen dan siapkan bahan yang diperlukan.
Putar sebentar botol RNase A dan tambahkan RNase A ke Buffer A1.
Encerkan DNA Wash Buffer sebagai berikut:
PD1812-00: Tambahkan 200 mL etanol 96-100% ke setiap botol sebelum digunakan.
PD1812-01: Tambahkan etanol 400mL 96-100% ke setiap botol sebelum digunakan.
PD1812-02: Tambahkan 500mL 96-100% etanol ke setiap botol sebelum digunakan.
Protokol Operasi
MORE INFO :
——————————————
Website : www.bmdstore.com
No Kantor : 021-2943 2095
No HP : 0813 1754 7882
Email : [email protected]
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