96-well Plasmid ezFilter Mini Kit 20×96 PD1812-02

96-well Plasmid ezFilter Mini Kit 20x96 PD1812-02 kualitas terbaik dan harga murah, produk tersertifikasi kalibrasi Akreditasi KAN. Produk terbaik hanya di BMDStore.COM

Product Description

Introduction

The yield of plasmid py number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 3 to 5 times. Please contact our customer service for further information and reference the table below for the commonly used plasmids.

Plasmid

Origin

High copy

Low copy

pACYC

P15A

10-12

pSC101

pSC101

5

pSuperCos

pMB1

DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high co

10-20

pBR322

pMB1

15-20

pUC

Muted pMB1

500-700

pGEMR

Muted pMB1

300-400

pBluescriptR

ColE1

300-500

Materials supplied by user

  •  Centrifuge with swing-bucket rotor (4,000 x g).
  •  Please disinfect 96-well 2.2 mL plates before use.
  •  Vacuum pump capable of achieving 300-400 mbar.
  •  Standard vacuum manifold.
  •  Oven or incubator preset to 70 oC.

Storage and Stability

Semua komponen dijamin selama 24 bulan sejak tanggal pembelian. Buffer A1 / RNase A harus disimpan pada 4oC.

Kit Contents

Catalog#

PD1812-S

PD1812-01

PD1812-02

Preps

1

4

20

96- Well 2.2 mLPlate

1

4

20

96- Well 1.6 mLPlate(Can be reused)

1

4

20

96-Well DNA Plate

1

4

20

96-Well Lysate Clearance Plate

1

4

20

96-Well Collection Plate

2

5

24

Ventilating Film

1

4

20

Sealing Film

4

16

80

Buffer A1

30 mL(Add RNase A before use)

110 mL(Add RNase A before use)

2 x 300 mL

Buffer B1

30 mL(Keep tightly capped after use)

110 mL(Keep tightly capped after use)

2 x 300 mL

Buffer N1

40 mL(Contain chaotropic salts)

160 mL(Contain chaotropic salts)

2 x 400 mL

DNA Wash Buffer

50 mL(Add 200 mL ethanol before use)

2×100 mL(Add 400 mL ethanol before use)

7 x125 mL(Add 500 mL ethanol before use)

Buffer KB

55 mL

240 mL

3 x 400 mL

Elution Buffer

25 mL

100 mL

450 mL

RNase A(20mg/mL)

160 μL

600 μL

2 x 1.5 mL

Use Manual

1

1

1

Before Starting

Ujilah buku pegangan ini dan kenali setiap langkahnya. Siapkan semua komponen dan siapkan bahan yang diperlukan.

Putar sebentar botol RNase A dan tambahkan RNase A ke Buffer A1.
Encerkan DNA Wash Buffer sebagai berikut:

PD1812-00: Tambahkan 200 mL etanol 96-100% ke setiap botol sebelum digunakan.

PD1812-01: Tambahkan etanol 400mL 96-100% ke setiap botol sebelum digunakan.

PD1812-02: Tambahkan 500mL 96-100% etanol ke setiap botol sebelum digunakan.

Protokol Operasi

MORE INFO :
——————————————
Website : www.bmdstore.com
No Kantor : 021-2943 2095
No HP : 0813 1122 7684 (WA)
Email : [email protected]

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